African Swine Fever Virus Genome Detection Using Real
Time Q Pcr Polymerase Chain Reaction Method-
Comparison of two Sample Specimen (Blood and Organs)
Larisa ANGHEL
(CIREASA)1,4, Maria-Virginia TANASA (ACRETEI)1, Natalia ROȘOIU1,2,3
1 Institute
of PhD Studies, Doctoral School of Applied Sciences Ovidiu s University
Constanta, cireasa.larisa-ct@ansvsa.ro
2 Prof.
Univ. Emeritus PhD Ovidiu s University, Faculty of Medicine
3 Academy of Romanian
Scientists
4 Molecular
Biology Laboratory, Veterinary Health and Food Safety Department D.S.V.S.A.
Constanta
Abstract. The study aims to show the result of CT (threshold
cycle) data collected over a three-year study conducted in the molecular
biology laboratory of D.S.V.SA Constanta using the Real Time Polymerase Chain
Reaction method to demonstrate the pathogenetic effect of African swine fever
virus during the replication and vascular changes present in all affected
organs.
Keywords: Polymerase
Chain Reaction (PCR), African Swine Fever virus
DOI https://doi.org/10.56082/annalsarscibio.2022.1.81